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Affinity Labeling Definition and Explanation PDF | Download eBooks

Learn Affinity Labeling definition in molecular biology with explanation to study “What is Affinity Labeling”. Study affinity labeling explanation with molecular biology terms to review molecular biology course for online degree programs.

Affinity Labeling Definition:

  • Labeling one substance by covalently attaching a reactive compound with specific affinity for the first substance (e.g., labeling the active site of an enzyme by linking a labeled substrate analog covalently to the enzyme)..

    Molecular Biology by Robert F. Weaver

Affinity Labeling Explanation:

Affinity labeling is a strategy to chemically modify an amino acid residue, within a ligand-binding site in an enzyme. This can happen either at a regulatory allosteric site or at the active site. The process involves a reagent that structurally resembles the natural ligand of the enzyme, but at the same time features a functional group that is capable of covalently reacting with many different amino acid residues.

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