What is Affinity Labeling in Molecular Biology? PDF | Download eBooks

Learn Affinity Labeling definition in molecular biology with explanation to study “What is Affinity Labeling”. Study affinity labeling explanation with molecular biology terms to review molecular biology course for online degree programs.

Affinity Labeling Definition

  • Labeling one substance by covalently attaching a reactive compound with specific affinity for the first substance (e.g., labeling the active site of an enzyme by linking a labeled substrate analog covalently to the enzyme)..

    Molecular Biology by Robert F. Weaver

Affinity Labeling Explanation

Affinity labeling is a strategy to chemically modify an amino acid residue, within a ligand-binding site in an enzyme. This can happen either at a regulatory allosteric site or at the active site. The process involves a reagent that structurally resembles the natural ligand of the enzyme, but at the same time features a functional group that is capable of covalently reacting with many different amino acid residues.

Keep Learning Molecular Biology Explanations

What are Abortive Transcripts?

Abortive transcripts are short RNA transcripts, about 6 nt long, that are produced at prokaryotic promoters. The release of such ...

What is Activator?

An Activator is a protein (transcription factor) that activates transcription by binding to an activator-binding region (also called an enhancer). ...

What is Alternative Splicing?

Alternative splicing or differential splicing is means splicing of the same pre-RNA in more than one ways, that in turn ...

What is Anticodon?

An anticodon is a sequence of three bases in a transfer RNA, that are complementary to a messenger RNA codon. ...

What is Archaea?

Archaea is the domain of single-celled organisms that are basically prokaryotes, but resemble to the eukaryotes and bacteria in terms ...

What is A-DNA?

A-DNA is a form of DNA, a biologically active double helical structure, which is assumed in solution by an RNA-DNA ...